Ethanol Fermentation on Mixed Sugars Using Mixed Culture of Two Yeast Strains  

Oleh:

Jasman ; Prijambada, Irfan Dwidya ; Hidayat, Chusnul ; Widianto, Donny

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 18 no. 02 (2013), page 116-122.
Topik: fermentation; ethanol; mixed culture; mixed sugar
Fulltext: 253-676-1-PB_Ros.pdf (158.68KB)

Isi artikel

The objective of this study was to evaluate the use of mixed cultures of the recommended yeast strains from a previous study on ethanol fermentation using a substrate mixture consisting of sucrose, glucose, and fructose. There were three mixed (combination) cultures namely OUT7096/OUT7913, OUT7096/OUT7921, and OUT7913/OUT7921. The fermentation medium contained sugar mixture consisting of glucose, fructose, and sucrose with a composition generally close to the composition of sugars in sweet sorghum juice. Overall, fermentation is carried out in two stages. First fermentation was performed using the three mixed cultures to determine the best combination based on the concentration of ethanol produced and the concentration of residual sugar. Second fermentation was conducted using the best mixed culture obtained from the fi rst stage. This second stage was intended to describe the pattern of the changes in the concentration of ethanol, sugars and biomass during the fermentation progresses and to determine some kinetic parameters namely ethanol yield (Yp/s), growth yield (Yx/s) and specifi c growth rate (µ). The results of the fi rst fermentation showed that the best mixed culture was OUT7913/OUT7921 and the subsequent fermentation using this culture provide the highest ethanol yield (Yp/s) = 0.47 g.g-1 that was reached at 53rd hour, growth yield (Yx/s) = 0.425 g.g-1, and µ = 0.12 hour-1.

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