Legume Nodulating Bacterium, Achromobacter xylosoxidans Found in Tropical Shrub Agroecosystem, Sumatera, Indonesia  

Oleh:

Wedhastri, Sri ; Fardhani, Dinar Mindrati ; Kabirun, Siti ; Widada, Jaka ; Widianto, Donny ; Evizal, Rusdi ; Prijambada, Irfan Dwidya

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 18 no. 02 (2013), page 161-167.
Topik: Legume Nodulating Bacteria; shrub agroecosystem; Achromobacter xylosoxidans; nodA; Glycine max
Fulltext: 252-672-1-PB_Ros.pdf (179.41KB)

Isi artikel

Legume nodulating bacteria (LNB), known also as rhizobia, are soil bacteria, which are able to form root nodules and fi x nitrogen in the leguminous plants. The LNB availability in the soil depends on the type of agroecosystem, where plant grows. In this study, we isolated LNB from the shrub agroecosystem in Sumatera, Indonesia, and obtained four selected bacterial strains. Among them, the isolate UGM48a formed root nodule in Macroptilium atropurpureum and showed highest number of nitrogenase activity. UGM48a also contains nifH and nodA genes. An analysis of the PCR-amplifi ed 16S rDNA and BLASTn analysis showed that UGM48a displayed 96% similarity with Achromobacter xylosoxidans. In addition, UGM48a were successfully nodulated Glycine max (L.) merr var. wilis. This is the fi rst report detecting A. xylosoxidans as nodule-forming species for Glycine max possesing the positive copy of nodA gene.

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