The Development of Pathogenicity of Avian Influenza Virus Isolated from Indonesia  

Oleh:

Wibowo, Michael Haryadi ; Srihanto, Eko Agus ; Putri, Khrisdiana ; Asmara, Widya ; Tabbu, Charles Rangga

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 18 no. 02 (2013), page 133-143.
Topik: avian infl uenza; haemagglutinin; cleavage site; basic amino acid; fusion site
Fulltext: 248-656-1-PB_Ros.pdf (197.69KB)

Isi artikel

Highly pathogenic avian infl uenza outbreak in Indonesia has been reported in various poultry due to H5N1 subtype. The presence of multiple basic amino acids within the cleavage site of HA glycoprotein has been identifi ed to be associated with the pathogenicity of avian infl uenza virus. The study was retrospective study which was designed to characterize the cleavage site and fusion site region of haemagglutinin gene of AIV isolated from various poultry in 2003 to 2013. Isolation, Identifi cation and propagation were carried out to collect viral stock. For virus detection, reverse transcriptase PCR (RT-PCR) method on H5 and N1 gene fragment was performed. All of RT-PCR HA gene positive products were sequenced for further nucleotide analysis and to determine the nucleotide composition at the targeted fragment. The results are all AIV isolates were identifi ed as H5N1 subtype. The sequence analyses revealed some motives of basic amino acid motive that were classifi ed as highly pathogenic avian infl uenza virus. Further analyses on fusion domain of all AIV isolated during the period 2003 to 2013 showed conserved amino acid.

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