Characterization of Streptomyces spp. Producing Indole-3-acetic acid as Biostimulant Agent  

Oleh:

Fretes, Charlie Ester de ; Sembiring, Langkah ; Purwestri, Yekti Asih

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 18 no. 02 (2013), page 83-91.
Topik: Streptomyces spp.; indole-3-acetic acid (IAA); indole-3-acetamide (IAM); Tryptophan Monooxigenase gene (iaaM)
Fulltext: 243-635-1-PB_Ros.pdf (267.02KB)

Isi artikel

Twenty six isolates of Streptomyces spp. obtained from Cyperus rotundus L. rhizosphere were tested for ability to produce indole-3-acetic acid (IAA) in yeast malt extract (YM) medium containing 2 mg/mL tryptophan. Screening of the isolates for ability to produce IAA was carried out by adding Salkowski reagent in bacteria culture and was measured quantitatively by spectrophotometer at ? 530 nm. Thin Layer Chromatography (TLC) method was used to determine IAA. To ensure the IAA production in Streptomyces isolates, gene involved in IAA biosynthesis was detected by amplifying Tryptophan Monooxigenase (iaaM) gene. The study of the effect of tryptophan on the production of IAA was measured at different concentrations of tryptophan (0, 1, 2, 3, 4, 5 mg/mL) in the bacterial culture. The result showed that there were two Streptomyces spp. isolates which could produce IAA, namely the isolates of Streptomyces sp. MS1 (125.48 µg/mL) and Streptomyces sp. BR27 (104.13 µg/mL). The TLC result showed that the compound in both isolates was identifi ed to be IAA. The amplifi cation results showed that iaaM gene was detected in both isolates. This results indicated that the IAM pathway is predicted involved in the biosynthesis of IAA in the selected isolates. Both of the isolates were able to produce IAA after 24 h incubation and the highest production was at 120 h incubation with the concentration of tryptophan was 2 mg/mL dan 1 mg/mL, respectively. Therefore, it is concluded that Streptomyces spp. isolates are able to produce IAA and potentially to be utilized as biostimulat agent.

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