Ability of Escherichia coli to produce hemolysis leads to a greater pathogenic effect on human sperm  

Oleh:

Boguen, Rodrigo ; Treulen, Favian ; Uribe, Pamela ; Villegas, Juana V.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 103 no. 05 (May 2015), page 1155–1161 .
Topik: Human spermatozoa; uropathogenic Escherichia coli; mitochondrial membrane potential; motility; reactive oxygen species

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K Non-tandon: tidak ada Tandon: 1

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Isi artikel

Objective To determine the effect on human sperm of Escherichia coli strains separated on the basis of their ability to produce hemolysis. Design Experimental study. Setting University-based laboratory. Patient(s) Semen samples from healthy donors. Intervention(s) Five million sperm, selected via the swim-up method, were incubated with 3 E. coli concentrations to obtain ratios of sperm to E. coli of 1:2, 1:16, and 1:128. The E. coli strains were: a hemolytic isolated strain (H), a nonhemolytic American Type Culture Collection strain (NH-ATCC), and a nonhemolytic isolated strain (NH-I). Main Outcome Measure(s) Aliquots of human sperm were used to measure progressive motility using computer-aided sperm analysis, mitochondrial membrane potential (??m) with a JC-1 (5,5',6,6' tetrachloro-1,1',3,3'-tetraethylbenzamidazolocarbocyanin iodide) and propidium iodide stain, and intracellular reactive oxygen species (iROS) with a dihydroethidium (DHE) stain. Sperm ??m and iROS were measured by flow cytometry. Sperm vitality was considered the mean of propidium iodide–negative and DHE-negative cells. Result(s) Sperm incubated with the H strain in a 1:2 sperm to bacteria ratio demonstrated a significant decrease in motility and ??m, and an increase of iROS. The NH-ATCC strain decreased sperm motility and ??m, but in a ratio of sperm to bacteria of 1:128; it increased iROS at a ratio of 1:16. The NH-I strain did not affect the analyzed sperm functions, even at a 1:128 sperm to bacteria ratio. Conclusion(s) Results show a greater pathogenic effect on human sperm of E. coli strains with, versus without, hemolytic capacity.

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