Influence of 17ß-estradiol treatment on the expression of NF-?B in complete hydatidiform mole culture  

Oleh:

Nurseta, Tatit ; Irwanto, Yahya ; Utami, Rahmi

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Medical Journal of Indonesia vol. 22 no. 04 (Nov. 2013), page 197-201.
Topik: 17-ß Estradiol; Hydatidiform mole; NF-?B
Fulltext: 599-1174-2-PB.pdf (474.78KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: M35.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Background: Genetic evidence has established a role of nuclear factor kappa B (NF-?B) signaling in oncogenesis. However, activity of NF-?B in complete hydatidiform mole (CHM) cell culture under 17ß-estradiol (E2) treatment is not yet known. Recently, a positive cross-talk between estrogen receptor (ER) and NF-?B to promote survival and progress of cancer cells to a more aggressive phenotype was established. In the present study, we examined the influence of E2 treatment on the NF-?B expression in CHM’s culture. Methods: This experimental study measured the expression of NF-?B in CHM culture treated with E2: 10, 100, 300, 600, and 1000 pg/mL and without E2. Imunohistochemistry staining was used to assess the expression of NF-?B. Microphotographs were taken using 400x magnification. Adobe photoshop CS2 was used to assess the NF-?B expression in cell nucleus. The lower the color intensity of cell RGBbv, is the higher the expression of NF-?B in cells. ANOVA test was performed to compare the expression of NF-?B. Results: NF-?B expression as indicated by color intensity in control group was 114.84 ± 9.02. NF-?B expression in E2 treatment groups were respectively: E2 10 pg/mL: 106.30 ± 13.95; E2 100 pg/mL: 82.47 ± 4.72; E2 300 pg/mL: 82.24 ± 2.67; E2 600 pg/mL: 69.05 ± 6.47; E2 1000 pg/mL: 68.49 ± 2.37. There was progressive decline in color intensity of cells with E2 treatment indicating the increase expression of NF-?B. Significant differences with the control group occurred in doses of E2 100, 300, 600, dan 1000 pg/mL. Conclusion: Treatment of CHM trophoblast culture with escalating doses of E2 was associated with the increase of NF-?B expression in a dose dependent manner.

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