Genetic Relatedness among Duku, Kokosan, and Pisitan in Indonesia Based on Random Amplifi ed Polymorphic DNA Markers  

Oleh:

Hanum, Laila ; Kasiamdari, Rina Sri ; Santosa ; Rugayah

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 17 no. 2 (Dec. 2012), page 121-131.
Topik: duku; kokosan; pisitan/langsat; genetic relatedness; RAPD
Fulltext: 141_Ros.pdf (299.12KB)

Isi artikel

Genetic relatedness among duku, kokosan, and pisitan from Indonesia were investigated using random amplifi ed polymorphic DNA (RAPD) markers. Eleven primers (OPA-01, OPA-02, OPA-10, OPB-07, OPB-11, OPB-12, OPB-15, OPT-16, OPU-14, OPU-19, and OPU-20) were used for amplifi cation and yielded a total of 174 DNA bands, of which 167 were polymorphic. Primer OPA-10, OPB-11, OPB-12, OPB-15, and OPU-19 produced all of the polymorphic DNA bands. The size of the amplifi ed DNA fragments ranged from 41-1546 bp. The dendrogram separated into two clusters at a genetic similarity coeffi cient of 0.76. The cluster 1 consisted of subclusters duku and several pisitan (pisitan OKI, pisitan Sleman, pisitan Hatu, pisitan Punggur, and pisitan Tanjung), and cluster 2 consisted of subclusters kokosan and pisitan. In the kokosan subclusters, including duku Drendan. Dendrogram supported the determination of taxonomic status of duku, kokosan, and pisitan as one species, namely Lansium domesticum Corr. and its divided into two groups, namely L. domesticum ’duku group’ and L. domesticum ’pisitan-kokosan group’. Thus, RAPD analysis was useful tool for determining the genetic variation and the genetics relatedness among duku, kokosan, and pisitan in Indonesia.

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