MicroRNA expression profiles in human testicular tissues of infertile men with different histopathologic patterns  

Oleh:

Abu-Halima, Masood ; Backes, Christina ; Leidinger, Petra ; Keller, Andreas ; Lubbad, Abdel Monem

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 101 no. 01 (Jan. 2014), page 78-86.
Topik: MicroRNA; small noncoding RNA; testis; nonobstructive azoospermia; male infertility

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2014.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To investigate the expression profiles of microRNA (miRNA) in human testes showing different histopathological patterns. Design Microarray with quantitative real-time polymerase chain reaction (qRT-PCR) validation. Setting University research and clinical institutes. Patient(s) Azoospermic men who underwent testicular biopsy for sperm recovery in preparation for intracytoplasmic sperm injection. Intervention(s) Testicular biopsies. Main Outcome Measure(s) Statistically significantly altered miRNA expression profiles among the testicular histopathologic patterns groups compared with normal pattern group. Result(s) According to miRNA array, a total of 197, 68, and 46 miRNAs were found to be differentially expressed when comparing the samples from Sertoli cell only (SCO), mixed atrophy (MA), and germ cell arrest (GA) groups, respectively, with normal spermatogenesis (N). Five miRNAs have been validated using qRT-PCR, and the results were consistent with miRNA array analysis. Bioinformatics analysis showed that five microRNAs (hsa-mir-34b*, hsa-mir-34b, hsa-mir-34c-5p, hsa-mir-449a, and hsa-mir-449b*) were involved in apoptosis, cell proliferation, and differentiation. Notably, potential target genes of these five miRNAs were involved in the spermatogenesis process. Conclusion(s) This study provides new insights into specific miRNAs that are expressed in infertile men with different histopathologic patterns, suggesting a role of miRNAs in regulating male germ and somatic cells and that their alteration is associated with reproductive abnormalities.

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