Endometrial integrin expression in women with recurrent implantation failure after in vitro fertilization and its relationship to pregnancy outcome  

Oleh:

Coughlan, Carol ; Sinagra, Melanie ; Ledger, William ; Li, T.C. ; Laird, Susan

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 100 no. 03 (Sep. 2013), page 825-830.
Topik: Implantation failure; endometrial receptivity; integrins

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2013.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To determine expression of integrins a1, a4, and aVß3 in the glandular and luminal epithelium, stroma, and cells in the blood vessel walls of the endometrium from women with recurrent implantation failure (RIF) and to determine if they are of prognostic value in determining pregnancy outcome. Design Prospective nonrandomized study. Setting Department of reproductive medicine. Patient(s) Forty-five women with RIF and six healthy fertile women were recruited. RIF was defined as the failure to conceive after the transfer of four good-quality embryos in three or more fresh or frozen cycles. Intervention(s) Endometrial biopsy samples were obtained from women with RIF and control women on days LH+7–LH+9 of the cycle. Expression of integrins a1, a4, and aVß3 was determined by immunohistochemistry. Main Outcome Measure(s) A semiquantitative measurement of expression of each integrin protein in the luminal and glandular epithelium, stroma, and cells in the blood vessel walls was determined by H-score analysis. Result(s) Expression of integrins a1 and a4 was greatest in the luminal and glandular epithelial cells and the cells in the blood vessel wall, and significantly higher expression of integrins a1 and a4 was seen in the glandular epithelium compared with the luminal epithelium (H-scores: a1 293 ± 15 and 180 ± 12, a4 287 ± 14 and 191 ± 11, respectively). Expression of aVß3 in the epithelium and blood vessels was also greater than in the stroma but there was no significant difference in expression of aVß3 in glandular and luminal epithelium. No significant difference in H-scores was seen for a1, a4, and aVß3 expression in any of the endometrial compartments in tissue from women with RIF and control women. No significant difference in a1, a4, and aVß3 expression in any compartment was observed between those who achieved a clinical pregnancy after subsequent assisted conception treatment (n = 21) and those who were unsuccessful (n = 24). Conclusion(s) RIF, when defined as failure to achieve a clinical pregnancy after the transfer of at least four good-quality embryos in three transfer cycles, is not associated with abnormal endometrial integrin expression. In addition, the expression of integrins a1, a4, and aVß3 appears to have no prognostic value in subsequent IVF treatment.

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