Role of angiotensin II and angiotensin-(1–7) in diabetes-induced oxidative DNA damage in the corpus cavernosum  

Oleh:

Kilarkaje, Narayana ; Yousif, Mariam H.M. ; El-Hashim, Ahmed Z. ; Makki, Batoul ; Benter, Ibrahim F.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 100 no. 01 (Jul. 2013), page 226-233.
Topik: Erectile dysfunction; renin-angiotensin-aldosterone system; DNA damage; angiotensin (1–7); AT1 receptor blockers; ACE inhibitors

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2013.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To investigate diabetes mellitus (DM)-induced oxidative DNA damage, putative involvement of angiotensin (Ang) II, and possible modulatory effects of Ang-(1–7) in rat corpus cavernosum (CC). Design In vivo study. Setting Research laboratory. Animal(s) Adult male Wistar rats. Intervention(s) Streptozotocin-induced diabetic rats received either captopril, losartan (both 300 mg/L in drinking water), or Ang-(1–7) (576 µg/kg/d IP) for a 3-week period immediately before sacrifice at 6 weeks of DM. Main Outcome Measure(s) Histopathological changes in CC were examined in Masson’s trichrome-stained tissue sections. Oxidative stress was evaluated by measuring total oxidant status and antioxidant status. The DNA damage was estimated by measuring 8-hydroxy-2'-deoxyguanosine by immunohistochemistry and ELISA. Result(s) The CC smooth muscle degeneration was observed in association with an increase in total oxidant status and a decrease in total antioxidant status in rats with DM. Oxidative DNA damage was significantly increased in both cytoplasm and nuclei of CC in DM. Treatment with captopril, losartan, or Ang-(1–7) inhibited these changes in rats with DM. Conclusion(s) The data indicate that Ang II signaling is involved in DM-induced structural changes and oxidative DNA damage in the CC and that modulation of the signaling by captopril, losartan, and Ang-(1–7) restores the effects of DM. Thus, Ang-(1–7)/MAS1 axis may be a novel therapeutic target for erectile dysfunction in DM.

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