Small glutamine-rich tetratricopeptide repeat–containing protein alpha is present in human ovaries but may not be differentially expressed in relation to polycystic ovary syndrome  

Oleh:

Butler, Miriam S. ; Yang, Xing ; Ricciardelli, Carmela ; Liang, Xiaoyan ; Norman, Robert J.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 99 no. 07 (Jun. 2013), page 2076-2083.
Topik: Ovary; PCOS; SGTA

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2013.04 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To evaluate the expression and function of small glutamine-rich tetratricopeptide repeat–containing protein alpha (SGTA), an androgen receptor (AR) molecular chaperone, in human ovarian tissues. Design Examine the effect of SGTA on AR subcellular localization in granulosa tumor cells (KGN) and SGTA expression in ovarian tissues. Setting University-based research laboratory. Patient(s) Archived tissues from premenopausal women and granulosa cells from infertile women receiving assisted reproduction. Intervention(s) None. Main Outcome Measure(s) AR subcellular localization and SGTA protein or mRNA levels. Result(s) SGTA and AR proteins were expressed in the cytoplasm of KGN cells and exposure to androgen stimulated AR nuclear localization. SGTA protein knockdown increased AR nuclear localization at low (0–0.1 nmol/L) but not high (1–10 nmol/L) concentrations of androgen hormone. In ovarian tissues, SGTA was localized to the cytoplasm of granulosa cells at all stages of folliculogenesis and in thecal cells of antral follicles. SGTA protein levels were similar when comparing primordial and primary follicles within core biopsies (n = 40) from women with and without polycystic ovary syndrome (PCOS). Likewise, SGTA mRNA levels were not significantly different in granulosa cells from preovulatory follicles after hyperstimulation of women with and without PCOS. Conclusion(s) SGTA is present in human ovaries and has the potential to modulate AR signalling, but it may not be differentially expressed

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali