Phaleria macrocarpa reduces glomerular growth factor expression in alloxan-induced diabetic rats  

Oleh:

Sulistyoningrum, Evy ; Setiawati

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI - non-atma jaya
Dalam koleksi: Universa Medicina vol. 32 no. 2 (May 2013), page 71-79.
Topik: Phaleria macrocarpa (Scheff.) Boerl.; growth factor expression; alloxan; diabetic nephropathy; rats
Fulltext: Phaleria macrocarpa.pdf (180.65KB)

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Perpustakaan FK Nomor Panggil: U01.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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BACKGROUND Diabetic nephropathy (DN) is the most serious complication of diabetes, causing end-stage renal disease throughout the world. Recent studies have reported a direct role of vascular endothelial growth factor (VEGF) and transforming growth factor-â (TGF-â) in DN pathogenesis. VEGF and TGF-â are expressed early in glomeruli in response to hyperglycemia. Active substances of Phaleria macrocarpa (PM) pericarp are known to have nephroprotective effects. This study aimed to evaluate the effects of Phaleria macrocarpa (Scheff.) Boerl pericarp extract on VEGF and TGF-â expression in alloxan-induced diabetic rats. METHODS An experimental study was conducted on twenty five male albino (Sprague Dawley) rats divided into five groups (of five each): normal control; diabetic; diabetic + metformin 100 mg/kgBW; diabetic + methanolic PM extract 250 mg/ kgBW; and diabetic + aqueous PM extract 250 mg/kgBW. Diabetes was induced by alloxan monohydrate 150 mg/BW intraperitoneally. Treatment was given for 3 weeks. VEGF and TGF-â expression analysis was performed by means of immunohistochemical technique. Differences between groups were assessed by one-way ANOVA. RESULTS VEGF expression in the PM extract group was significantly lower than that in the diabetic group and even metformin group (p<0.01). TGF-â expression in methanolic PM extract group was significantly lower than in diabetic and metformin group (p<0.01), but aqueous PM extract group only showed significancy when compared with diabetic group (p< 0.01). CONCLUSIONS Phaleria macrocarpa pericarp extract reduces glomerular expression of TGF-â and VEGF in alloxan-induced diabetic rats.

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