Detection of Carbapenemase Encoding Genes in Enterobacteriace, Pseudomonas aeruginosa, and Acinetobacter baumanii Isolatedfrom Patients at Intensive Care Unit Cipto Mangunkusumo Hospital in 2011  

Oleh:

Karuniawati, Anis ; Saharman, Yulia R. ; Lestari, Delly C.

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Acta Medica Indonesiana vol. 45 no. 2 (Apr. 2013), page 101-106.
Topik: Enterobacteriaceae; P. aeruginosa; A. baumanii; carbapenemase encoding genes.
Fulltext: Detection of Carbapenemase Encoding Genes in Enterobacteriace.pdf (1.81MB)

Ketersediaan

Perpustakaan FK Nomor Panggil: A02.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Aim: to determine the prevalence of carbapenemase encoding genes (blaIMP-1, blaVIM-2, blaKPC-2, blaOXA-48, and blaNDM-1) of carbapenem-resistant Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumanii isolated from the intensive care unit patients as pathogens, in Cipto Mangunkusumo hospital (ICU-RSCM) in 2011. Methods: we examined the carbapenemase encoding genes in the clinical microbiology laboratory (LMK FKUI/RSCM). Duplex- and simplex PCR methods were conducted to detect the resistant genes. Results: we found 4 (5%) P. aeruginosa strains carry blaIMP-1 gene and all were isolated from sputum specimens. The prevalence of carbapenem resistant among Gram-negative bacilli isolated from ICU-RSCM, are Enterobacteriaceae 27.6%, P. aeruginosa 21.9%, and A. baumannii 50.5%. The New Delhi Metallo-ß-lactamase encoding gene (blaNDM-1) was detected in 1 K. pneumonia isolated from sputum as well. The other genes, i.e. blaKPC-2, blaVIM-2, and blaOXA-48 were not found in any isolates. The absence of other genes indicated that other mechanisms may play a role in the occurrence of carbapenem resistance in pathogens isolated in ICURSCM. Conclusion: this study confirmed that the prevalence of carbapenems resistant Gram-negative bacilli in ICU-RSCM in 2011 was high. The carbapenemase encoding genes, which were detected among the carbapenems resistant Gram-negative bacilli, were blaIMP-1 and blaNDM-1.

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