Distribution of Dimethylarginine-Dimethylaminohydrolase-II (DDAH2)Gene Polymorphism in Hemodialysis Patients  

Oleh:

Thaha, Muhammad ; Yusuf, Muhammad ; Empitu, Maulana A. ; Bakarman, Achmad

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Acta Medica Indonesiana vol. 45 no. 2 (Apr. 2013), page 83-88.
Topik: CKD; DDAH2 gene polymorphism; hemodialysis; hypertension.
Fulltext: Dimethylarginine-Dimethylaminohydrolase-II (DDAH2).pdf (1.64MB)

Ketersediaan

Perpustakaan FK Nomor Panggil: A02.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Aim: to describe the distribution of single nucleotide polymorphisms (SNPs) at -449 (promoter region) DDAH 2 in hemodialysis (HD) patients. Methods: this study was a descriptive study, 56 HD patients and 30 healthy individuals were enrolled. Based on its etiology, the HD patient group was further divided into hypertension (HT) group and non-HT group. DNA was extracted from whole blood samples with a commercially available DNA isolation kit. Genotyping of the polymorphisms was performed using PCR-based SNP detection methods (Applied Biosystems, Carlsbad, USA) based on 5’-exonuclease activity assays for rs805305 (-449 G/C). Allelic variation was assessed in each participant. Results: heterozygotes were observed as the most abundant genotypes in both groups (70% in healthy individuals and 55% in HD patients), followed by GG genotype in the HD patients (30%), while CC (27%) was the second most common genotype polymorphism in the healthy individuals. Conclusion: there is a significant difference in distribution of DDAH 2 gene polymorphism among HD patients compared to healthy individuals (p=0.01).

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