Does early cell death cause germ cell loss after intratesticular tissue grafting?  

Oleh:

Van Saen, Dorien ; Goossens, Ellen ; Aerts, Joeri L. ; Haentjens, Patrick ; Tournaye, Herman

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 99 no. 05 (Apr. 2013), page 1264-1272.
Topik: ANDROLOGY; Annexin V; cell death; fertility preservation; intratesticular grafting; TUNEL

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2013.04 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To assess cell death in intratesticular grafts. Design Experimental animal study. Setting University. Animal(s) F1-hybrids from SV129 X C57BL. Intervention(s) Intratesticular tissue transplantation was performed and cell death in the grafts was evaluated at different time points after transplantation. Main Outcome Measure(s) Apoptotic cell death in spermatogonia was evaluated by flow cytometry with the use of the annexin V assay. Immunohistochemistry was used to evaluate graft development and the global occurrence of cell death. Result(s) The highest level of spermatogonia-specific cell death was found on days 4 and 10, although no statistical difference was observed compared with control tissue. Statistically significant reductions in tubule integrity were observed 1 day and 2 months after transplantation. More degenerated tubules were observed in the center of the grafts 1 and 4 days after transplantation, and higher numbers of apoptotic tubules were found 1 day after transplantation. No difference in overall cell death was observed between grafts and controls for any time point except for the frozen grafts 1 day after transplantation. Conclusion(s) Spermatogonia-specific apoptosis does not explain the stem cell loss observed after intratesticular tissue grafting; it probably results from degeneration of tubules in the center of the graft owing to hypoxia during the first days after transplantation.

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