Reversin Increase the Plasticity of Bone Marrow-derived Mesenchymal Stem Cell for Generation of Cardiomyocyte in Vitro  

Oleh:

Pikir, Budi S. ; Susilowati, Helen ; Hendrianto, Eryk ; Abdulrantam, Fedik

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Acta Medica Indonesiana vol. 44 no. 01 (Jan. 2012), page 23-27.
Topik: Bone marrow-derived mesenchymal stem cells; cardiomyocyte; reversin; plasticity; 5-aza-2’-deoxycytidine.
Fulltext: Reversin Increase the Plasticity of Bone.pdf (1.44MB)

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Perpustakaan FK Nomor Panggil: A02.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Aim: to speed up transdifferentiation of bone marrow-derived stem cells into cardiomyocyte in vitro by inducing dedifferentiation of bone marrow-derived mesenchymal stem cell, before induction by 5-aza-2’- deoxycytidine into cardiomyocyte. Methods: two-three months old 2.5 kg weight adult male New Zealanad Rabbits were anesthezied with ether, thigh bones were excised, and bone marrow cells were obtained by aspiration. Results: in our experiments after 1 week of mesenchymal stem cell cultures, 20 nM reversin was given to induce dediferentiation and after 24 hours exposure with 9 mM 5-aza-2-deoxycytidine, early phase of cardiomyocyte differentiation appeared as cultured cell were strongly positive for GATA-4 and weakly positive for MLC-2a, although beating cardiomyocyte has not yet appeared at the end of experiment. These experiments also showed a marked CD34+ and c-kit+ gene expression on RT-PCR examination. Conclusion: reversine increase plasticity of bone marrow-derived mesenchymal stem cell to generate cardiomyocyte after 5-aza-2’-deoxycytidine induction. CD34+ and c-kit+ may be a marker for cardiomyocyte progenitor cells

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