Isolation and Cultivation of Mesenchymal Stem Cells from Iliac Crest Bone Marrow for Further Cartilage Defect Management  

Oleh:

Lubis, Andri M.T. ; Sandhow, Lakshmi ; Lubis, Vita K. ; Noor, Alfiah ; Gumay, Febrilian ; Merlina, Maurin ; Yang, Wenny ; Lorensia, Vinci

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Acta Medica Indonesiana vol. 43 no. 3 (Jul. 2011), page 178-184.
Topik: Mesenchymal stem cells; bone marrow; isolation; cultivation.
Fulltext: Isolation and Cultivation of Mesenchymal Stem Cells.pdf (2.05MB)

Ketersediaan

Perpustakaan FK Nomor Panggil: A02.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Aim: to validate isolation and cultivation methods of bone marrow mesenchymal stem cells (BM-MSCs) from iliac crest, and to compare biological characteristics of BM-MSCs from different age groups for preparation of autologous stem cell therapy in cartilage defect.Methods: patients undergoing spinal surgery were selected and grouped according to age. Iliac crest bone marrow from the patients was aspirated. BM-MSCs were isolated from the bone marrow and then cultivated. Their biological characteristics including morphological appearances and surface biomarkers were evaluated. Growth curves were observed. Sterility and Mycoplasma tests were also performed for quality assessment of BM-MSCs culture procedure.Results: in average, cultivated-BM-MSCs reached the number of 7.56-22.95 x 106 in 4-7 weeks period. BM-MSCs of all age groups showed the same quality of morphology, shape and surface biomarkers (CD105+, CD73+, CD34-, CD45-, CD14-, CD19-, HLA-DR-). Conclucion: our procedures in isolating and cultivating of BM-MSCs have reached required amount for implantation into the cartilage lesion. In addition, the cultivated-BM-MSCs’ biological characteristics were also in accordance with International Society of Cell Therapy (ISCT) MSCs criteria.

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