Efek Sitotoksik Ekstrak Etanol Sarang Semut (Myrmecodia pendens) Pada Sel Line Kanker Serviks HeLa Uji Eksperimental Secara In Vitro Cytotoxic Effect of Ethanolic Extract of Sarang Semut (Myrmecodia pendens) on HeLa Cervix Cancer Cell Line  

Oleh:

Fatmawati, Dina ; Puspitasari, Prista Karina ; Yusuf, Iwang

Jenis: Article from Journal - ilmiah nasional - tidak terakreditasi DIKTI
Dalam koleksi: Sains Medika (Jurnal Kedokteran dan Kesehatan) vol. 03 no. 02 (Jul. 2011), page 112-120.
Topik: HeLa cervix cancer cell line; cytotoxic effect; ethanolic extract of sarang semut(Myrmecodia pendens).
Fulltext: Efek Sitotoksik Ekstrak Etanol.pdf (262.19KB)

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Perpustakaan FK Nomor Panggil: S11.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Background: Sarang semut (Myrmecodia pendens) is a traditional plant that has been known to containanti cancer components. Flavonoids and tannins were contained in sarang semut plant which are believedhas cytotoxic effect against cancer cell line. This study aims at cytotoxic effect ethanolic extract of sarangsemut at various concentrations on HeLa cervical cancer cell line.Design and Method: The method was quasi experimental with post test only non equivalent control groupdesign. HeLa cell was divided into two groups. The first group as positive control with doxorubicin, secondgroup as treatment with ethanolic extract of sarang semut at various concentrations. Ethanolic extract ofsarang semut concentrations used were 3,91 µg/ml; 7,81 µg/ml; 15,63 µg/ml; 31,25 µg/ml; 62,50 µg/ml;125 µg/ml; 250 µg/ml; 500 µg/ml; 1000 µg/ml. Cytotoxic effect was evaluated by direct counting methodwith tryphan blue dye then using probit regression analysis to find IC50 value.Result: Inhibitory concentration 50 (IC50) value ethanol extract of sarang semut was 33,28 µg/ml. Ethanolextract of sarang semut had a cytotoxicity effect categorized as the moderately active (20 ìg/ml< IC50< 100ìg/ml). Inhibitory concentration 50 (IC50) value doxorubicin was 5,56 µg/ml. Cytotoxicity effect of doxorubisinhigher than cytotoxicity effect of ethanolic extract of sarang semut.Conclusion: Ethanolic extract of sarang semut (Myrmecodia pendens) had a cytotoxic effect categorized asthe moderately active on HeLa cell (Sains Medika, 3(2):112-120).

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