Increases Interleukin (IL)-4 Expression is Related with Decreases of Bax and Lymphocyte Apoptosis in Bronchiolus and Lung of Asthmatic Mice  

Oleh:

Yuliarto, Saptadi ; Kusuma, HMS Chandra ; Widjajanto, Edi

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Jurnal Kedokteran Brawijaya vol. 25 no. 03 (Dec. 2009), page 100-110.
Topik: Asthma; mice; ovalbumin; lymphocyte apoptosis; IL-4; lymphocyte-Bax

Ketersediaan

Perpustakaan FK Nomor Panggil: J34.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Decrease of lymphocyte apoptosis is one factor that leads to chronic airway inflammation in allergic asthma. Previous studies revealed the role of interleukin (IL)-4 in preventing lymphocyte apoptosis; however there are few studies about the role of lymphocyte-Bax and its relationship with IL-4 in asthma. The aim of the study is to demonstrate IL-4 role in preventing lymphocyte apoptosis via lymphocyte-Bax inactivation in bronchioli and lungs of asthmatic mice. It is a randomized control group experimental study. The subject was Balb/c mice that categorized into 2 groups: asthma and non-asthma. Asthma group was sensitized by ovalbumin intraperitoneally in day 0 and 14, followed by inhalation every 2-3 days for 6 weeks. In week 8, all of mice were terminated. The IL-4 and lymphocyte-Bax expression was examined through immunohistochemistry method, whereas lymphocyte apoptosis by TUNEL method. Independent sample t-test, Mann Whitney U test, regression analysis, and path analysis was used in statistical analysis with confident interval 95%. The bronchioli and lungs specimens were obtained from 18 mice (9 in each group). Lymphocyte apoptosis was similar between 2 groups (p=0.116), additionally lymphocyte-Bax decreased in asthmatic group (p=0.003). This indicated low activity of lymphocyte apoptosis in asthmatic group. Conversely, IL-4 expression increased in asthmatic group (p=0.00). There was moderate negative correlation (r2=0.29, p=0.011) between IL-4 and lymphocyte apoptosis. There was also strong negative correlation (r*=0.39, p=0.002) between IL-4 and lymphocyte-Bax expression. However, we did not find any correlation between lymphocyte-Bax and lymphocyte apoptosis (r=0.37, p=0.065). Path analysis revealed the stronger direct relationship between IL-4 and lymphocyte apoptosis rather than indirect relationship via lymphocyte-Bax inactivation. We conclude that increases of IL-4 expressions inhibit lymphocyte apoptosis; however, it is not due to the Bax-lymphocyte inactivation.

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