Progestin-induced heart and neural crest derivatives expressed transcript 2 is associated with fibulin-1 expression in human endometrial stromal cells  

Oleh:

Cho, Hisayuu ; Okada, Hidetaka ; Tsuzuki, Tomoko

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 99 no. 01 (Jan. 2013), page 248-255.
Topik: REPRODUCTIVE BIOLOGY; Endometrial stromal cells; decidualization; FBLN1; HAND2; progestin

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2013.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To investigate whether heart and neural crest derivatives expressed transcript 2 (HAND2) regulates fibulin-1 (FBLN1) expression during decidualization of human endometrial stromal cells (ESCs). Design In vitro experiment. Setting Research laboratory. Patient(s) Twenty-four patients undergoing hysterectomy for benign reasons. Intervention(s) ESCs were cultured with various progestins (medroxyprogesterone acetate [MPA], norethisterone, levonorgestrel, dienogest, and P), E2, dexamethasone, and/or 8-bromoadenosine 3', 5'-cyclic monophosphate (8-Br-cAMP). HAND2 and FBLN1 were silenced by small interfering RNA technology. Main Outcome Measure(s) HAND2 and FBLN1 expression levels were assessed by real-time polymerase chain reaction and Western blot analysis. Result(s) MPA or E2 + MPA increased HAND2 mRNA levels in ESCs in a time- and dose-dependent manner, and this stimulatory effect was blocked by RU-486 (P receptor antagonist). HAND2 was increased by E2 + MPA earlier than FBLN1. Simultaneous MPA and 8-Br-cAMP treatment synergistically enhanced HAND2 mRNA levels. P and all the progestins significantly increased HAND2 mRNA levels, whereas E2, 8-Br-cAMP, or dexamethasone alone had no effect. Silencing of HAND2 expression significantly reduced FBLN1 expression, whereas FBLN1 silencing had no effect on HAND2 expression. Conclusion(s) These results suggest that progestin-induced HAND2 contributes to FBLN1 expression in human ESCs.

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