Epithelial cell protein milk fat globule–epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometrium  

Oleh:

Riggs, Ryan M. ; Bocca, Silvina ; Anderson, Sandra ; Franchi, Anahi

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 98 no. 06 (Dec. 2012), page 1549-1556.
Topik: REPRODUCTIVE BIOLOGY; Apoptosis; caspase activity; endometrium; hCG; human; MFG-E8

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2012.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To study the regulation of apoptosis in human endometrial cells. The specific aims were to determine whether milk fat globule–epidermal growth factor 8 (MFG-E8), a novel endometrial epithelial protein, modulates caspase activation and DNA fragmentation; and to examine whether hCG, an early embryonic product, regulates Bax and Bcl-2 equilibrium, as well as MFG-E8 expression. Design Primary cultures of human endometrial epithelial cells (EECs) and endometrial stromal cells (ESCs). Setting Academic center. Patient(s) Ovulatory women aged 21–30 years. Intervention(s) Treatment with MFG-E8 and hCG. Main Outcome Measure(s) Apoptotic activity was quantified using a luciferase assay. Deoxyribonucleic acid fragmentation was detected by TUNEL assay. Bax, Bcl-2, and MFG-E8 messenger RNA expression levels were determined by quantitative reverse transcription–polymerase chain reaction. Immunocytochemistry was used to establish cell purity and presence of MFG-E8 and hCG-R (receptor) proteins. Result(s) Endometrial epithelial cells were cytokeratin+, vimentin-, MFG-E8+, and hCG-R+, whereas ESC were vimentin+, cytokeratin-, MFG-E8-, and hCG-R+. Treatment of ESC with MFG-E8 resulted in a 13-fold increase in caspase activity and a 30-fold increase in TUNEL. On the other hand, hCG decreased messenger RNA expression of Bax in ESC. Conclusion(s) Milk fat globule–epidermal growth factor 8 has proapoptotic activity, suggesting participation in endometrial remodeling via an epithelial–stromal cell paracrine effect. Conversely, pregnancy levels of hCG has opposite effects on stromal cells.

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