Human Skin Fibroblast Telomeres are Shortened after Ultraviolet Irradiation  

Oleh:

Ma, H-M. ; Liu, W. ; Zhang, P. ; Yuan, X-Y.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The Journal of International Medical Research vol. 40 no. 05 (Sep. 2012), page 1871-1877.
Topik: Photoageing; Ultraviolet Irradiation; Cellular Senescence; Telomere Length; Real-Time Polymerase Chain Reaction

Ketersediaan

Perpustakaan FK Nomor Panggil: J11.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

OBJECTIVE: Telomere length was used as a biomarker of cell senescence to explore the role of telomere shortening in photoageing induced by ultraviolet A (UVA) light. METHODS: Real-time polymerase chain reaction was used to determine telomere length in cultured human fibroblasts of different generations and after exposure to UVA at doses up to 10 000 mJ/cm2. Twoway analysis of variance was used to determine whether passaging or UVA was the main factor contributing to telomere shortening. RESULTS: In nonirradiated cells, telomere length was inversely related to cell generation number. In fibroblasts exposed to UVA at a dose of 1000 or 10 000 mJ/cm2, telomere length was significantly shorter than that of nonirradiated controls and was negatively related to UVA dose. CONCLUSIONS: Telomere length and subsequent cell viability may be affected by UVA irradiation. DNA damage caused by UVA irradiation may initiate the photoageing process and telomeres may be a useful new target for attempts to prevent photoageing.

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