Efek Pentagamavunon-0 Terhadap Konsentrasi cAMP dan Prugesteron pada Kultur Sel Luteal yang Mengandung Teofilin  

Oleh:

Purwaningsih, Endang ; Soejono, Sri Kadarsih ; Dasuki, Djaswadi ; Meiyanto, Edy

Jenis: Article from Journal - ilmiah nasional - tidak terakreditasi DIKTI
Dalam koleksi: Jurnal Kedokteran YARSI vol. 17 no. 02 (May 2009), page 93-100.
Topik: signal transduction; curcumin analog; culture plate; ELISA; RIA
Fulltext: J51 v17 n2 2009 p93.pdf (3.63MB)

Ketersediaan

Perpustakaan PKPM Nomor Panggil: J51 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk
Perpustakaan FK Nomor Panggil: J26.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk

Isi artikel

Curcumin analog (Pentagamavunon-0/PGV-0) can inhibit steroidogenesis of luteal cell culture. Corpus luteum secretes progesterone by LH stimulation. The main transduction signal of luteal cells steroidogenesis is through the cAMP/PKA. The objective of this study was to know the effect of PGV-0 on cAMP and progesterone concentration of luteal cell culture containing theophylline. The subject was corpus luteum of rat Sprague Dawley strain induced with PMSG (10 IU). PGV-0 was given shortly after the stimulation of LH and or PGF2a with or without theophyline. The cell culture then put into the incubator for 24 hours. Concentration of cAMP was assessed by ELISA whereas the progesterone concentration was determined by RIA. The result showed that LH stimulation caused cAMP and progesterone increase significantly. The inhibition of PGF2a on cAMP and progesterone concentration showed no significant difference compared to the control. Theophyllines increased the cAMP and progesterone concentrarion by LH stimulation. PGV-0 inhibited the progesterone concentration by LH stimulation. In conclusion, PGV-0 inhibits signal transduction of lutheal cell in down stream cAMP.

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