Novel metabolites and roles for a-tocopherol in humans and mice discovered by mass spectrometry–based metabolomics  

Oleh:

Johnson, Caroline H ; Slanar, Ondrej ; Krausz, Kristopher W. ; Dong, Wook Kang

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The American Journal of Clinical Nutrition vol. 96 no. 04 (Oct. 2012), page 818-830 .
Topik: VITAMINS; Minerals; phytochemicals; xenobiotic use

Ketersediaan

Perpustakaan FK Nomor Panggil: A07.K.2012.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Background: Contradictory results from clinical trials that examined the role of vitamin E in chronic disease could be a consequence of interindividual variation, caused by factors such as xenobiotic use. Cometabolism of vitamin E with other pharmaceutical products could affect the bioavailability of the drug. Thus, it is necessary to understand fully the metabolic routes and biological endpoints of vitamin E. Objective: The objective was to uncover novel metabolites and roles of vitamin E in humans and mouse models. Design: Human volunteers (n = 10) were fed almonds for 7 d and then an a-tocopherol dietary supplement for 14 d. Urine and serum samples were collected before and after dosing. C57BL/6 mice (n = 10) were also fed a-tocopherol–deficient and –enriched diets for 14 d. Urine, serum, and feces were collected before and after dosing, and liver samples were collected after euthanization. Ultraperformance liquid chromatography electrospray ionization time-of-flight mass spectrometry and multivariate data analysis tools were used to analyze the samples. Results: Three novel urinary metabolites of a-tocopherol were discovered in humans and mice: a-carboxyethylhydroxychroman (a-CEHC) glycine, a-CEHC glycine glucuronide, and a-CEHC taurine. Another urinary metabolite, a-CEHC glutamine, was discovered in mice after a-CEHC gavage. Increases in liver fatty acids and decreases in serum and liver cholesterol were observed in mice fed the a-tocopherol–enriched diet. Conclusion: Novel metabolites and metabolic pathways of vitamin E were identified by mass spectrometry–based metabolomics and will aid in understanding the disposition and roles of vitamin E in vivo.

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