Early Detection and Serotyping of Dengue Viruses by Using Reverse Transcription Polymerase Chain Reaction (RT-PCR) 2 Primers  

Oleh:

Siregar, Abdul Rahman ; Wibawa, Tri ; Wijayanti, Nastiti

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 16 no. 2 (2011), page 71-75.
Topik: Dengue Virus; DHF; Early Detection; Serotyping; RT-PCR 2 Primers.
Fulltext: 145-437-1-PB_thom.pdf (605.91KB)

Isi artikel

Recently several methods for confirming dengue virus infection have been developed such as virus isolation, detection of virus antigen, and nucleic acid detection by using RT-PCR method. It has been reported that rapid detection method to confirm dengue virus infection by using Multiplex RT-PCR had been successfully developed. It was reported more effective than the other methods with 100% sensitivity and specivicity at the early phase (day 1-3) of infection. This study was aimed to develop rapid detection and serotyping methods for dengue virus using RT-PCR 2 primers (Dcon and preM). The whole blood samples were collected from suspected dengue fever patients that had been confirmed with NS1 detection kit from during Februari-August 2009. The PCR products showed that in 12 samples, 100% were positive with different pattern among the serotypes especially for DEN1 and DEN2, but not for DEN3 and DEN4. This method was also able to confirm the double infection DEN2-DEN3, but not for the other ones because of the unspecific pattern. It is indicated that the RT-PCR 2 primers was a promising method for early detection and serotyping dengue virus infection.

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