Antifungal Production of a Strain of Actinomycetes spp Isolated from the Rhizosphere of Cajuput Plant: Selection and Detection of Exhibiting Activity Against Tested Fungi  

Oleh:

Alimuddin ; Widada, Jaka ; Asmara, Widya ; Mustofa

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 16 no. 1 (2011), page 1-10.
Topik: Rhizosphere; Cajuput; Streptomyces; Antifungal
Fulltext: 6-22-1-PB_thom.pdf (687.07KB)

Isi artikel

Actinomycetes are bacteria known to constitute a large part of the rhizosphere micro-biota. Their isolation is an important step for screening of new bio-active compounds. Culturable actinomycetes populations from cajuput plant rhizosphere soils in Wanagama I Forest UGM Yogyakarta were collected to study about their anti-fungal activity. Among 17 of a total 43 isolates that showed activity were screened for producing antifungi substances. Screening for anti-fungal activity of isolates were performed with dual culture bio-assay in vitro. One isolate that was designated as Streptomyces sp.GMR-22 was the strongest against all tested fungi and appeared promising for a sources of anti-fungal. Culture’s supernatant and mycelia were extracted with chloroform, ethyl acetate and methanol, respectively. Antifungal activity of crude extracts was tested by diffusion method against tested fungi. The result indicates that isolates of actinomycetes from cajuput plant rhizosphere could be an interesting sources of anti-fungal bio-active substances.

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