Purification and Characterization of Streptomyces sp. IK Chitinase  

Oleh:

Margino, Sebastian ; Nugroho, A. Joko

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 15 no. 1 (2010), page 29-36.
Topik: Chitinase; Streptomyces; Purification; Characterization 1*; 2and Widya Asmara2
Fulltext: 30-75-1-PB_thom.pdf (436.12KB)

Isi artikel

Streptomyces sp. IK isolated from compost inoculants, could produce extra cellular chitinase in a medium containing 0.2% (w/v) colloidal chitin, fermented for 96 hours at 30oC. The enzyme was purified by a combination of ammonium sulphate precipitation and DEAE-Cellulose anion-exchange chromatography. On SDS-polyacrylamide gel electrophoresis analysis, the purified enzyme showed a mass of 71 kDa. Chitinase was optimally active at pH of 6.7 and at 37oC. Km value and Vmax of the protein for colloidal chitin were 2.92 mg/ml and 4.26 ìg/h, respectively.

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