ß-Carotene Supplementation Decreases Placental Transcription of LDL Receptor-Related Protein 1 in Wild-Type Mice and Stimulates Placental ß-Carotene Uptake in Marginally Vitamin A-Deficient Mice  

Oleh:

Wassef, Lesley ; Shete, Varsha ; Hong, Alice

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: JN: The Journal of Nutrition vol. 142 no. 08 (Jul. 2012), page 1456-1462.
Topik: NUTRIENT; Nutrient Physiology; Metabolism; Nutrient-Nutrient Interactions

Ketersediaan

Perpustakaan FK Nomor Panggil: J42.K.2012.02 Non-tandon: 1 (dapat dipinjam: 1) Tandon: tidak ada

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Isi artikel

The human diet contains ß-carotene as the most abundant precursor of vitamin A, an essential nutrient for embryogenesis. Our laboratory previously showed the importance of ß-carotene metabolism via ß-carotene-15,15'-oxygenase (CMOI) to support mouse embryonic development. However, the mechanisms regulating embryonic acquisition and utilization of ß-carotene from the maternal circulation via placenta remain unknown. We used wild-type (WT) and Lrat-/-Rbp-/- (L-/-R-/-) mice, the latter being a model of marginal vitamin A deficiency. Pregnant dams, fed a nonpurified diet sufficient in vitamin A throughout life, were i.p. supplemented with ß-carotene or vehicle at 13.5 d postcoitum (dpc). Effects of this acute maternal supplementation on retinoid and ß-carotene metabolism in maternal (serum, liver) and developing tissues (placenta, yolk sac, embryo) were investigated at 14.5 dpc. We showed that, upon supplementation, placental ß-carotene concentrations were greater in L-/-R-/- than in WT mice. However, the retinoid (retinol and retinyl ester) concentrations remained unchanged in placenta (and in all other tissues analyzed) of both genotypes upon ß-carotene administration. We also showed that upon a single i.p. ß-carotene supplementation, placental LDL receptor-related protein (Lrp1) mRNA expression was lower in WT mice, and embryonic CmoI mRNA expression was greater in L-/-R-/- mice. Together, these data suggest a potential role of LRP1 in mediating the uptake of ß-carotene across the placenta and that even a marginally impaired maternal vitamin A status may influence uptake and utilization of ß-carotene by the placenta and the embryo.

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