Arachidonic acid regulation of the cytosolic phospholipase A2a/cyclooxygenase-2 pathway in mouse endometrial stromal cells  

Oleh:

Zhao, Zhen-Ao ; Zhang, Zhi-Rong ; Xu, Xiu ; Deng, Wen-Bo

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 97 no. 05 (May 2012), page 1199-1205.
Topik: REPRODUCTIVE BIOLOGY; Uterus; embryo implantation; decidualization; arachidonic acid

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2012.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To investigate the role of arachidonic acid (AA) in mouse endometrial stromal cells. Design Experimental animal study. Setting University research laboratory. Animal(s) Sexually mature female CD1-strain mice. Intervention(s) Primary culture of endometrial stromal cells. Main Outcome Measure(s) Western blot and real-time polymerase chain reaction for gene expression and/or phophorylation analysis. Luciferase assay for Cox-2 promoter analysis. Result(s) AA-derived prostaglandins play important roles during embryo implantation and decidualization. However, the function of AA itself in reproduction is largely unknown. In this study, exogenous AA stimulated cPLA2a phosphorylation and COX-2 expression, mainly through ERK1/2 in mouse endometrial stromal cells, and p38 inhibitor modestly inhibited cPLA2a phosphorylation induced by AA. The induction of COX-2 by AA was diminished by short interfering RNA against C/EBPß and inhibitory C/EBPß (LIP). C/EBPß binding site at -872–-864 of Cox-2 promoter contributes to Cox-2 promoter activation induced by C/EBPß transfection. The expression of C/EBPß protein induced by AA was inhibited by p38 inhibitor, and the phosphorylation of C/EBPß induced by AA was inhibited by p38 inhibitor and ERK1/2 inhibitor. A nonmetabolized analogue of AA (ETYA) also enhanced cPLA2a phosphorylation and COX-2 expression. The activation of cPLA2a/COX-2 by AA was not inhibited by COX inhibitor indomethacin. Conclusion(s) AA can induce cPLA2a/COX-2 pathway activation in mouse endometrial stromal cells.

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