| The prevalences of inflammation-related diseases, such as asthma, hypertension, stroke, osteoarthritis, acute and chronic hyperuricemia, rheumatoid arthritis, and cancer, are still quite high. The bioactive compounds of Sargassum sp. have the potential as an antioxidant and anti-inflammatory agent. This study aimed to measure the total phenolic and flavonoid content of Sargassum sp. ethyl acetate extract from Ria Beach, Dompu Regency, West Nusa Tenggara, and to evaluate its antioxidant and anti-inflammatory activity. Sargassum sp. was macerated in ethyl acetate (1:4 w/v) for 24 hours, then concentrated using a rotary evaporator (40ºC). The total phenol and flavonoid contents were measured using the colorimetric method. The antioxidant and anti-inflammatory activities were evaluated using DPPH and carrageenan-induced paw edema models, respectively. The results demonstrated the weak antioxidant of Sargassum sp. with an IC50 value of 605,24 ± 26,53 µg/mL. Its total phenolic and flavonoid contents were 103,013 ± 0,479 mg GAE/g and 20,572 ± 0,089 mg QE/g, respectively. The percentages of inflammatory inhibition of Sargassum sp at doses of 200, 400, and 800 mg/kg BW were 53,80 ± 4,68%, 62,35 ± 4,05%, and 60,90 ± 2,88%, respectively. Based on its AUC value, it showed a significant difference (p<0.05) compared to the negative control. This study concluded that Sargassum sp. ethyl acetate extract at the dose of 400 mg/kg BW had the highest anti-inflammatory activity. |