Detail Bioprospecting Potential of Bacteria Isolated from Honey and Bee-Associated Products from Indonesia (presented at the 2018 International Meeting of the Federation of Korean Microbiology Societies) Bibliografi Author: Magdalena, Stella ; Purnawidjaja, Ivana Topik: JABFUNG-FTB-STM-2023-17 Bahasa: (EN )     Tahun Terbit: 2018     Jenis: Poster Fulltext: B17_Poster FKMS 2018 PRINT.pdf (280.69KB; 3 download) Abstract Many recent studies support the link between symbiotic and pathogenic microbial interactions in gut microbiota of honey bee. However, our knowledge of the honey microbial community still remain largely unknown. The present study was designated to evaluate the occurrence of bacteria and yeasts in honey and bee-associated products from Indonesia. A total of 115 isolates were isolated from 24 samples. The supernatants were used to screening against fungal pathogenic (Aspergillus fumigatus ATCC 204305) by using dual assay method; Candida albicans ATCC 10231 and bacteria (Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923) by using well diffusion methods. More of the isolates shown antimicrobial activity against fungal pathogenic compared than bacteria. Eighty four out of 115 isolates (73.04%) exhibited enzymatic activity on at least one of the assay. Protease activity is the most exhibited activity shown by the isolates (62.61%) followed by amylase (42.61%) and lipase (41.74%). Six isolates (L30, L35, Y12, Y33, Y21, and Y23) were selected to characterize by using 16S rDNA sequencing. Molecular identification of the isolates performed that L30 and L35 were identified as B. subtilis; Y12 and Y33 were B. velezensis; and Y21 and Y23 were B. amyloliquefaciens. These data can be explored further to be applied as an alternative way to test the purity of honey. The results demonstrated that isolates with high antimicrobial and enzymatic activity had potential application as biopreservation agents and probiotic. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

Copyright © 2006, 2007 Unika Atma Jaya, all rights reserved