Detail Analysis of Endophyte Bacterial Community from Healthy Banana Plant Grown in Fusarium oxysporum Infected Area in Bali and West Java, Indonesia (presented at 32nd Symposium of the Malaysian Society for Microbiology (MSM 2014) 6-8 December 2014 Terengganu Equestrian Resort, Malaysia) Bibliografi Author: Karmawan, Listya Utami ; Dwivany, Fenny Martha ; Aryantha, I Nyoman Pugeg Topik: JABFUNG-FTB-LUK-2022 Bahasa: (EN )     Penerbit: Malaysian Society for Microbiology (MSM) and Universiti Malaysia Terengganu     Tempat Terbit: Terengganu    Tahun Terbit: 2014     Jenis: Others (lainnya) Fulltext: Ganjil 2014_IntSeminar_SMSM.pdf (4.8MB; 4 download) Abstract Fusarium wilt (Panama disease) caused by fungus Fusarium oxysporum, is known as the most destructive disease in banana. Conventional management of Fusarium wilt usually focuses on fumigation and finding new resistant cultivar of banana. Yet, research on optimising susceptible plant growth and defense system was neglected until the recent decades. Currently it is known that plants obtain benefits via mutualistic relationship with endophytes, which induces plant growth or systemic resistance to pest or disease. Endophyte isolation from healthy banana plant grown in Fusarium infected area would be beneficial for microbial bioprospecting of Fusarium wilt control. A total 129 isolates of bacterial endophyte were isolated from different tissue parts of healthy pisang kepok (Musa balbisiana, BBB group) in Buleleng, Bali, and pisang ambon kuning (Musa acuminata, AAA group) in Subang-Punclut, West Java, Indonesia. These isolates were clustered using enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR). Partial 16S rRNA gene sequencing was performed on isolates with unique ERIC-PCR bands. The major phlya of endophyte isolates in Buleleng, Bali were Firmicutes (40.0%), Proteobacteria (40.0%), Bacteriodetes (17.8%), and Actinobacteria (2.2%). The major phyla of endophyte isolates in Subang-Punclut, West Java were Proteobacteria (62.2%), Actinomycetes (24.4%), and Firmicutes (13.3%). Bacterial community was also different in different parts of plants. The most common genus isolated from banana grown in Buleleng, Bali were Bacillus and Sphingobacteria (18.5% each) in roots, Bacillus (50%) in cormus/ stem, Bacillus (60%) in pseudostem, and Bacillus (60%) in leaf. The most common genus in healthy banana roots, cormus/stem, pseudostem, and leaf in Subang-Punclut, Jawa Baral, Bali were Burkholderia (20%), Staphylococcus (25%), Pseudomonas (38.5%), Micrococcus and Pseudomonas (33.3% each) respectively. This study provides valuable endophyte isolates that can be further screened to find useful isolates for Fusarium wilt control. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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