Purification and Carbohydrate Analysis of Recombinant Human Erythropoietin Expressed in Yeast System Pichia Pastoris  

Oleh:

Santoso, Adi ; Wardiana, Andri

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Makara Seri Sains vol. 15 no. 1 (Apr. 2011), page 75-78.
Topik: EPO; Gel Filtration Chromatography; His-Trap Affinity Chromatography; Monosaccharide
Fulltext: 888-1808-1-SM.pdf (301.62KB)

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Perpustakaan Pusat (Semanggi) Nomor Panggil: MM65 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

For clinical purposes, pure protein and identification of carbohydrate structure from recombinant erythropoietin are needed. Purification was done by Immobilized Metal Affinity Chromatography (IMAC) column charged with Ni2+ (His-Trap affinity chromatography) and continued with gel filtration chromatography column to get purer protein. The carbohydrate group which is oligosaccharide from the resulting pure protein then can be recognized by using N- and O-glycosidase. Pure oligosaccharide was hydrolyzed to produce various monosaccharide through incubation with 4 N HCl in 100 oC temperature for 6 hours and the result was applied on High Performance Liquid Chromatography (HPLC) column to learn the composition of its monosaccharide.

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