Detail Isolation and characterization of pathogenic Escherichia coli bacteriophages from chicken and beef offal (article of BMC Research Notes Vol.13 (2020), Article number: 8) Bibliografi Author: Lukman, Celosia ; Yonathan, Christopher ; Magdalena, Stella ; Waturangi, Diana Elizabeth Topik: Phyllosphere; Quorum sensing; Violacein; JABFUNG-FTB-DNW-2021-09 Bahasa: (EN )     Penerbit: Springer Nature     Tahun Terbit: 2020     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: 9 Isolation Characterization Pathogenic E coli Bacteriophages.pdf (1.04MB; 2 download) B2_Artikel BMC-Bacteriophage Celo.pdf (1.03MB; 1 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract Objective This study was conducted to isolate and characterize lytic bacteriophages for pathogenic Escherichia coli from chicken and beef offal, and analyze their capability as biocontrol for several foodborne pathogens. Methods done in this research are bacteriophage isolation, purification, titer determination, application, determination of host range and minimum multiplicity of infection (miMOI), and bacteriophage morphology. Results Six bacteriophages successfully isolated from chicken and beef offal using EPEC and EHEC as host strain. Bacteriophage titers observed between 109 and 1010 PFU mL-1. CS EPEC and BL EHEC bacteriophage showed high efficiency in reduction of EPEC or EHEC contamination in meat about 99.20% and 99.04%. The lowest miMOI was 0.01 showed by CS EPEC bacteriophage. CI EPEC and BL EPEC bacteriophage suspected as Myoviridae family based on its micrograph from Transmission Electron Microscopy (TEM). Refers to their activity, bacteriophages isolated in this study have a great potential to be used as biocontrol against several foodborne pathogens. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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