General imprinting status is stable in assisted reproduction–conceived offspring  

Oleh:

Feng, Chun ; Tian, Shen ; Yu Zhang ; Jing, He

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 96 no. 06 (Dec. 2011), page 1417-1423.
Topik: Assisted reproductive technology (ART); epigenetic; genomic imprinting; methylation; offspring

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To evaluate whether the genomic imprinting status of assistant reproductive technology (ART)–conceived offspring is stable. Design Prospective clinical observational study. Setting In vitro fertilization (IVF) center, university-affiliated teaching hospital. Patient(s) Sixty ART-conceived babies (30 IVF and 30 intracytoplasmic sperm injection [ICSI]) and 60 naturally conceived babies. Intervention(s) Collection of umbilical cord blood and peripheral blood samples. Main Outcome Measure(s) Expression profile was examined by microarray and real-time reverse-transcription polymerase chain reaction (PCR), allele-specific expression was studied by direct sequencing after PCR, and DNA methylation status was investigated by sodium bisulfite sequencing. Result(s) Hierarchic clustering demonstrated no obvious clustering between the ART- and naturally conceived offspring, suggesting similar genomic imprinting expression between the two groups. Three differentially expressed genes were identified in ART-conceived offspring, with PEG10 and L3MBTL up-regulated and PHLDA2 down-regulated. Allele-specific expression of the differentially expressed imprinted genes was maintained in the majority of the ART- and naturally conceived offspring. However, in one ICSI case, monoallelic expression of L3MBTL was disrupted and all CpGs were completely unmethylated. These were not inherited from the parents. Conclusion(s) The global profile of imprinting is stable in children conceived through ART. However, imprinting of a few specific imprinted genes may be vulnerable in a fraction of ART-conceived children.

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