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Potential of Rhizopus delemar ATH53 in Determining the Quality of Tempeh: Vitamin B12, Antioxidant, Antibacterial, and Flavor (presented at The 2nd International Microbial Biotechnology Conference Jakarta, 15-16 November 2016)
Bibliografi
Author:
Barus, Tati
;
Hartanti, Anastasia Tatik
;
Lawa, Maya Agnesia Wie
Topik:
Antioxidant
;
Antibacterial
;
Organoleptic
;
Rhizopus delemar ATH53
;
Tempeh
;
Vitamin B12
;
JABFUNG-FTB-ATH-2021-12
Bahasa:
(EN )
Penerbit:
Fakultas Teknobiologi Unika Atma Jaya (Faculty of Biotechnology Atma Jaya Catholic University of Indonesia)
Tempat Terbit:
Jakarta
Tahun Terbit:
2016
Jenis:
Poster
Fulltext:
12 - 3 Poster Potential1 2.pdf
(1.46MB;
5 download
)
Abstract
Tempeh is a fermented soybean food from Indonesia. According to previous research, R. microsporus is mostly used in Indonesian tempeh. R. delemar also can be used as fermentation agent in tempeh but the amount in Indonesia has reduced. Therefore the aim of this research is for examining the potential of R. delemar ATH53 in determining the quality of tempeh such as antioxidant, vitamin BI2, antibacterial activity, and flavor. Tempeh was prepared with pure culture of R. delemar AHT53, Raprima and Pengrajin yeast. The antibacterial activity was tested with diffusion disc method, antioxidant activity was tested with DPPH method and the amount of vitamin B12 was measured by High Performance Liquid Chromatography (HPLC). The flavor of each tempeh was tested with hedonic method by panelists. The result shows that there is no antibacterial activity from each tempeh. The highest antioxidant activity was found in tempe with R.delemar ATH53 (50.43%), followed by tempeh with Ragi Raprima (42.98%) and tempeh with Ragi Pengrajin (36.91%). The highest vitamin B12 amount was found in tempeh with "Raprima" (26.71 ppm) and followed by tempeh with R. delemar ATH53 (21.72 ppm) and tempeh with "Pengrajin" yeast (15.28 ppm). In organoleptic test, the result shows that tempeh with R. delemar ATH53 is the most acceptable tempeh, followed by tempeh with "Raprima" and "Pengrajin" yeast.
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