PELABELANANTIBODI ANTI-NSI DENGUE KELINCI DENGAN HORSERADISH PEROKSIDASE  

Oleh:

Dewi, Beti E. ; Sudiro, T. Mirawati ; Paisal

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Media Penelitian dan Pengembangan Kesehatan vol. 20 no. 04 (Dec. 2010), page 173.
Topik: Protein NS1; Dengue anti-NSl IgG; Labeling; HRP

Ketersediaan

Perpustakaan PKPM Nomor Panggil: M45 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk
Perpustakaan FK Nomor Panggil: M32.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk

Isi artikel

Dengue NS1 protein can be an ideal target for early detection of dengue virus infection. The aim of the study is to label rabbit anti-NS1 antibody with HRP, so that it can be used for detection of NSI protein. The design of this study is laboratory experimental. Anti-NS1 IgG-contained rabbit serum was purified with column chromatography (Sephadex G-200). The result of purification was labeled with HRP using periodate method. Then, HRP-labeled IgG was generated with dot blot and ELISA. Using dot blot assay, wefound that rabbit anti-NS1 IgG labeled with HRP is successful. Nevertheless, the ability of detection was not so good (1:1600). In addition, HRP-labeled IgG used to detect NS1 protein utilizing ELISA resulted in high negative control absorbance (0,453 ::I:0:,013). Therefore, we cannot interpret the assay. The labeling was successful, but it need further optimatization in order to get the HRP-labeled IgG can be used in ELISA. Optimatization was also needed to increasing the ability of detection of HRP-labeled IgG in dot blot assay.

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