Effect of curcumin and pentagamavunon-0 on the viability of cultured luteal cells  

Oleh:

Purwaningsih, Endang

Jenis: Article from Journal - ilmiah nasional - tidak terakreditasi DIKTI
Dalam koleksi: Jurnal Kedokteran YARSI vol. 17 no. 01 (Jan. 2009), page 45.
Topik: Effect of Curcumin; Pentagamavunon-0; Cultured Luteal Cells
Fulltext: J51 v17 n1 2009 p45.pdf (225.63KB)

Ketersediaan

Perpustakaan PKPM Nomor Panggil: J51 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk
Perpustakaan FK Nomor Panggil: J26.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk

Isi artikel

he purpose of this study was to investigate the effect of curcumin and pentagamavunonn-0 (PGV-0) on the viability of cultured luteal cells (percent of viable cells). The cultured luteal cells were obtained from corpus luteum of induced 12 immature Sprague Dawley rats by a single 10 IU Pregnat Mare’s Serum Gonadotropin. Subject in this study was 4 days old corpus luteum which was cultured in Minimal Essential Medium (MEM) containing 10% Fetal Bovine Serum. This experiment consisted of 12 groups, e.g. one group as control (solvent), three groups as control with LH and or PGF2a stimulation, and eight groups treated with 100 µM curcumin and PGV-0 in three replicates. Evaluation was conducted after 24 hours of treatment by observation of viable cells with MTT method. The result showed that the viability of cultured luteal cells with curcumin or PGV-0 only was not significantly different compared to control goups. However, curcumin and PGV-0 on cultured luteal cells stimulated by LH and or PGF2a showed significant differences compared to control or LH and PGF2a only.

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