Lysine requirement of the enterally fed term infant in the first month of life  

Oleh:

Huang, Lisha ; Hogewind-Schoonenboom, Jacomine E. ; de Groof, Femke ; Twisk, Jos W. R.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The American Journal of Clinical Nutrition vol. 94 no. 06 (Dec. 2011), page 1496-1503 .
Topik: Nutritional status; Dietary Intake; and Body Composition

Ketersediaan

Perpustakaan FK Nomor Panggil: A07.K.2011.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Background: Infant nutrition has a major impact on child growth and functional development. Low and high intakes of protein or amino acids could have a detrimental effect. Objective: The objective of the study was to determine the lysine requirement of enterally fed term neonates by using the indicator amino acid oxidation (IAAO) method. l-[1-13C]phenylalanine was used as an indicator amino acid. Design: Twenty-one neonates were randomly assigned to lysine intakes that ranged from 15 to 240 mg · kg-1 · d-1. Breath, urine, and blood samples were collected at baseline and during the plateau. The mean lysine requirement was determined by using biphasic linear regression crossover analysis on the fraction of 13CO2 recovery from l-[1-13C]phenylalanine oxidation (F13CO2) and phenylalanine oxidation rates calculated from the l-[1-13C]phenylalanine enrichment of urine and plasma. Results: The mean (±SD) phenylalanine flux calculated from urine and plasma l-[1-13C]phenylalanine enrichment data were 88.3 ± 6.9 and 84.5 ± 7.4 µmol · kg-1 · h-1, respectively. Graded intakes of lysine had no effect on phenylalanine fluxes. The mean lysine requirement determined by F13CO2 was 130 mg · kg-1 · d-1 (upper and lower CIs: 183.7 and 76.3 mg · kg-1 · d-1, respectively). The mean requirement was identical to the requirement determined by using phenylalanine oxidation rates in urine and plasma. Conclusions: The mean lysine requirement of enterally fed term neonates was determined by using F13CO2 and phenylalanine oxidation rates calculated from the l-[1-13C]phenylalanine enrichment of urine and plasma. These methods yielded a similar result of 130 mg lysine · kg-1 · d-1. This study demonstrates that sampling of 13CO2 in expired air is sufficient to estimate the lysine requirement by using the IAAO method in infants.

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