Notch 1 signaling pathway effect on implantation competency  

Oleh:

Chu, Po-Wei ; Wang, Yi-Pei ; Chen, I-Ching ; Pan, Hsiu-Min ; Wu, Gwo-Jang

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 96 no. 05 (Nov. 2011), page 1225-1229.
Topik: Embryo implantation; Notch 1 signaling pathway; Matrigel invasion assay

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To investigate the relationship between the Notch 1 signaling pathway and the embryo implantation rate. Design Mouse embryos were cultured in vitro, and implantation competency was quantified. Setting Tertiary fertility center of a university teaching hospital. Animal(s) Outbred ICR strain mouse embryos. Intervention(s) The expression of Notch 1 was altered by adding a ?-secretase inhibitor to the culture medium. We quantified the consequent effect on embryo implantation. Main Outcome Measure(s) We measured the messenger RNA level of Notch 1 gene at different embryonic stages, embryo implantation rate under different culture conditions, the amount of Notch 1, and related implantation competency. Result(s) Quantitative polymerase chain reaction showed that the expression of Notch 1 increased during the implantation window. Adding ?-secretase inhibitor in the culture medium decreased the percentage of blastocysts in a dose-dependent manner. A Matrigel invasion assay showed that the competency of implantation required adequate expression of Notch 1 intracellular domain. Conclusion(s) Expression of Notch 1 at the proper time is required for the competency of embryo implantation; this effect is mediated through regulation of Notch 1 intracellular domain expression.

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