Mesenchymal stem cells derived from Wharton jelly of the human umbilical cord ameliorate damage to human endometrial stromal cells  

Oleh:

Yang, Xiaoqing ; Zhang, Mu ; Zhang, Yuquan ; Li, Wei

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 96 no. 04 (Oct. 2011), page 1029-1036.
Topik: Mesenchymal stem cells (MSCs); endometrium; damage; mifepristone

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To investigate the effect of mesenchymal stem cells isolated from Wharton jelly of umbilical cord (WJ-MSCs) on ameliorating damaged human endometrial stromal cells (ESCs). Design Experimental study. Setting University-affiliated hospital. Patient(s) Sixteen endometrial tissues were obtained from women undergoing hysterectomy. Eight umbilical cords were obtained from full-term deliveries. Intervention(s) ESCs were cultured with mifepristone to get damaged ESCs, then damaged ESCs were co-cultured with WJ-MSCs. Main Outcome Measure(s) The proliferation of ESCs was investigated by Cell Counting Kit 8, and the percentage of apoptosis by annexin-V–fluorescein isothiocyanate binding. The mRNA and protein expression of vascular endothelial growth factor (VEGF) and caspases 3, 8, and 9 were determined by one-step quantitative real-time polymerase chain reaction and Western blot. Result(s) After exposure to mifepristone, the proliferation of ESCs decreased and the apoptosis percentage increased in a dose- and time-dependent manner. At a certain dose and duration, this damage continued even after the withdrawl of mifepristone at 48 hours. When the damaged ESCs were cocultured with WJ-MSCs, the proliferation of these damaged cells was significantly increased and apoptosis percentage decreased. In addition, the level of VEGF mRNA and protein decreased and that of caspases 3, 8, and 9 increased. Conclusion(s) WJ-MSCs may serve as a promising treatment approach to ameliorate endometrial damage.

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