Background: Diabetic wound has a long process of wound healing. There are few effective therapies to treat diabetic wound. Platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are autologous products rich in growth factors that can increase neovascular and collagen in diabetic wound healing. The aim of this study is to compare the effectivity between administration of PRP and PRF, and is expected to be used as a reference for diabetic wound treatment.
Methods: This experimental study used 36 male Wistar rats, 12 weeks old. Diabetes was induced in Wistar rats (n=24) by intra peritoneal administration of alloxan (150mg/kg). The incision wound was located at 4 cm dorsolateral from the vertebral column, 2 cm long with 2 mm depth on dorsal vertebrae. A total 36 male Wistar rat were divided into 6 groups. The wound tissues in Group A (negative control), Group B (PRP), and Group C (PRF) were excised on day 7. On the other hand, Group D (negative control), Group E(PRP), and Group F (PRF) were excised on day 14. The skin tissues were made into Hematoxylin and Eosin (HE) histologic slides and Masson Trichrome (MT) slides. Neovascularization was measured with light microscope and Collagen was measured using ImageJ.
Result: Data was analyzed using one-way ANOVA post hoc, and the results obtained showed that administration of PRP on 7 and 14 day wound age, neovascular (p <0,05) and collagen (p <0,05). Administration of PRF on 7 and 14 day wound age, neovascular (p <0,05) and collagen (p > 0,05). The comparison of wound healing treated with PRP and PRF on 7 day wound age, neovascular (p > 0,05) and collagen (p < 0,05). The comparison of wound healing treated with PRP and PRF on 14 day wound age, neovascular (p > 0,05) and collagen (p > 0,05)
Conclusion: This study suggests that PRP and PRF promotes neovascular and dermal collagen synthesis in diabetic rat wound healing had a significant effect. |