Genome-wide identification of micro-ribonucleic acids associated with human endometrial receptivity in natural and stimulated cycles by deep sequencing  

Oleh:

Sha, Ai-Guo ; Liu, Ji-Long ; Jiang, Xiao-Ming ; Ren, Jian-Zhi

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 96 no. 01 (Jul. 2011), page 150-155..
Topik: Deep sequencing; endometrial receptivity; microRNA; menstrual cycle

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.04 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To identify microRNAs (miRNAs) associated with endometrial receptivity. Design Observational study. Setting Medical center. Patient(s) Healthy, regularly cycling women undergoing IVF treatment. Intervention(s) Gonadotropin stimulation and endometrial biopsy. Main Outcome Measure(s) Quantification of miRNA expression profiles by deep sequencing. Result(s) The miRNA expression profiles in human endometrium on days LH+2 and LH+7 (LH = 0 is the day of the LH surge) in natural cycles as well as on days hCG+4 and hCG+7 (hCG = 0 is the day of hCG injection) in stimulated cycles were determined by deep sequencing. In natural cycles, there were 20 significantly changed miRNAs in human endometrium on LH+7 compared with LH+2. These miRNAs were predicted to target a large set of genes with different functions, including cell cycle, transport, cell adhesion, cell death, and metabolism. In stimulated cycles, 22 miRNAs were significantly dysregulated on hCG+7 in comparison with LH+7, 11 of which exhibited putative estrogen response elements or P response elements in the promoters. Additionally, unsupervised hierarchical clustering analysis demonstrated that the miRNA expression profile on hCG+4 was similar to that on LH+7, suggesting that ovarian stimulation may alter the window of endometrial receptivity. Conclusion(s) MiRNAs may be novel biomarkers for human endometrial receptivity and may help optimize the protocol for IVF treatment.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali