Optimal timing of oocyte maturation and its relationship with the spindle assembly and developmental competence of in vitro matured human oocytes  

Oleh:

Yang, Yu ; Jie, Yan ; Liu, Zi C. ; Yan, Li Y. ; Li, Min

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 96 no. 01 (Jul. 2011), page 73-78.
Topik: Culture duration; embryo quality; human in vitro matured oocytes; parthenogenetic activation; spindle assembly

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.04 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To develop and test a simple, feasible approach to improve spindle assembly and developmental competence of human in vitro matured oocytes by parthenogenetic activation in maturation medium. Design Prospective, randomized study. Setting University research laboratory-based assisted reproductive technology laboratory. Patient(s) Four hundred thirty-two patients with male factor infertility undergoing intracytoplasmic sperm injection (ICSI) cycles. Intervention(s) In vivo failed-to-mature oocytes from the ICSI cycles were divided into four groups according to differing duration after the extrusion of first polar body (0–1, 2–3, 4–5, and 8–9 hours). Oocytes spindles in each group were immunostained for a-tubulin and chromosomes, and observed by confocal microscopy. Main Outcome Measure(s) Rate from pronuclear stage to blastocyst, embryo grading at the eight-cell and blastocyst stages, and spindle assembly. Result(s) There was a statistically significantly higher rate of development at the eight-cell and blastocyst stages in the 2- to 3-hour and 4- to 5-hour groups. The grading results at the eight-cell and blastocyst stages also showed that the proportion of embryos of high quality was similar among the 2- to 3-hour and 4- to 5-hour groups, and in these groups it was statistically significantly higher than the 0- to 1-hour and 8- to 9-hour groups. The results of immunofluorescence demonstrated that there was a statistically significantly higher rate of normal spindle assembly in the 2- to 3-hour and 4- to 5-hour groups. Conclusion(s) Optimal timing of maturation benefits the development of competence of in vitro matured oocytes by promoting normal spindle assembly.

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