What is the best frame rate for evaluation of sperm motility in different species by computer-assisted sperm analysis?  

Oleh:

Castellini, Cesare ; Dal Bosco, Alessandro ; Ruggeri, Silvia ; Collodel, Giulia

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 96 no. 01 (Jul. 2011), page 24-27.
Topik: Computer-assisted sperm analysis; CASA; frame rate; sperm motility; track speed

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.04 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To evaluate the effect of acquisition rate in the computer-assisted sperm analysis (CASA) of sperm motility with different kinetic characteristics (linearity and track speed) belonging to different species. Design Prospective research study. Setting Andrology laboratory of department of applied biology. Patient(s) Semen samples from human fertile donors, rabbit, bull, and ram. Intervention(s) Semen analysis by optical microscopy and sperm motility evaluation with the use of CASA. Main Outcome Measure(s) Analysis of sperm tracks with a CASA system acquired at different frame rates (FRs) (12–200 Hz). Result(s) The FR had no effect on the motility rate or on the sperm track reconstruction, whereas all the other kinetic values were affected by sampling frequency. Sperm with low speed (category b) and/or linear trajectories (category a) are less affected by sampling frequency and do not require particularly high FR to be correctly evaluated. On the contrary, fast nonlinear sperm (category c) would require a very high frequency to fully describe the path. Conclusion(s) Because all the commercial CASA systems acquire at an FR of 30–60 Hz, an increase in FR is required to render the estimation results closer to the “real path” mainly for fast nonlinear sperm.

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