Tobacco consumption and benzo(a)pyrene-diol-epoxide–DNA adducts in spermatozoa: in smokers, swim-up procedure selects spermatozoa with decreased DNA damage  

Oleh:

Perrin, Jeanne ; Tassistro, Virginie ; Mandon, Marion ; Jean-Marie, Grillo

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 95 no. 06 (May 2011), page 2013-2017.
Topik: DNA adducts; spermatozoa; polycyclic aromatic hydrocarbon; sperm selection technique; swim-up

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To analyze the distribution of benzo(a)pyrene-diol-epoxide (BPDE)–DNA adducts in spermatozoa selected and nonselected by a swim-up procedure with relation to smoking habits. Design Comparative study. Setting Public university and public university hospital. Patient(s) Seventy-nine men (37 smokers and 42 nonsmokers) who visited an infertility clinic for diagnostic. Intervention(s) Tobacco and environmental exposure assessment, semen sample analysis, swim-up procedure, BPDE-DNA adduct immunolabeling. Main Outcome Measure(s) BPDE-DNA adduct quantification in selected (SEL-SPZ) and nonselected (NONSEL-SPZ) spermatozoa. Data were normalized by using a normalized fluorescence value (NFV). Result(s) The mean NFV (±SD) in SEL-SPZ was significantly higher in smokers than in nonsmokers (18.9 ± 11.5 vs. 10.5 ± 10.4, respectively). Within smokers, a paired analysis (SEL-SPZ and NONSEL-SPZ) showed that NFV was significantly lower in SEL-SPZ than in NONSEL-SPZ (20.0 ± 11.3 vs. 31.5 ± 16.0, respectively). Conversely, within nonsmokers, the mean NFV was higher in SEL-SPZ than in NONSEL-SPZ (10.3 ± 10.6 vs 4.3 ± 7.1, respectively). Conclusion(s) Tobacco consumption is associated with BPDE-DNA adducts in spermatozoa. In smokers, semen processing by swim-up recovers potentially fertilizing spermatozoa that show a significantly lower amount of BPDE-DNA adducts compared with NONSEL-SPZ. Further study is needed to improve the spermatozoa selection in smoking patients requiring assisted reproductive technologies.

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