Evaluation of DNA methylation status at differentially methylated regions in IVF-conceived newborn twins  

Oleh:

Li, Lei ; Wang, Liya ; Le, Fang ; Liu, Xiaozhen

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 95 no. 06 (May 2011), page 1975-1979.
Topik: Assisted reproductive technology; ART; differentially methylated regions; DMRs; in vitro fertilization; IVF; twins

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To examine the effect of assisted reproductive technology on the stability of DNA methylation at differentially methylated regions (DMRs) in twins conceived by IVF. Design Prospective clinical observational study. Setting IVF center, university-affiliated teaching hospital. Patient(s) Fifty-nine pairs of twins were recruited, including 29 pairs conceived through IVF and 30 pairs of naturally conceived twins. Intervention(s) Collection of umbilical cord blood samples. Main Outcome Measure(s) DNA was extracted from umbilical cord blood. Two maternally methylated regions (KvDMR1 and PEG1) and one paternally methylated region (H19/IGF2 DMR) were analyzed using bisulfite-based technologies. Result(s) Although H19/IGF2 DMR and KvDMR1 showed slightly more variable levels of methylation in IVF cases than in spontaneous cases, methylation indices did not reveal significant differences at three DMRs between IVF-conceived and naturally conceived twins. Conclusion(s) Our results suggest no significant increase in imprint variability at these DMRs, but the greater variance in the IVF twins has a biologically meaningful consequence and may be a topic for future investigation. Large cohorts are needed to systematically assess the potential epigenetic risk in twins conceived with IVF.

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