Interleukin (IL)11 mediates protein secretion and modification in human extravillous trophoblasts  

Oleh:

Sonderegger, Stefan ; Yap, Joanne ; Menkhorst, Ellen ; Weston, Gareth

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Human Reproduction vol. 26 no. 10 (Oct. 2011), page 2841-2849.
Topik: REPRODUCTIVE BIOLOGY; Interleukin 11 (IL11); Glucose-Regulated Protein 78 (GRP78); Protein Disulfide Isomerase Family A; Member 3 (PDIA3) STAT3; Proteomic; Extravillous Trophoblast (EVT
Fulltext: Hum. Reprod.-2011-Sonderegger-2841-9.pdf (348.13KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: H07.K.2011.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

BACKGROUND Human trophoblast invasion and differentiation are essential for a successful pregnancy outcome. Dysregulation of these processes can lead to placental pathologies such as pre-eclampsia. The molecular mechanisms; however, are poorly understood. Interleukin (IL)11—a cytokine that regulates endometrial epithelial cell adhesion, trophoblast motility and invasion during implantation—may be involved in some of these processes. METHODS AND RESULTS The effect of IL11 on protein expression was investigated in trophoblastic HTR8/SVneo cells and primary extravillous trophoblasts (EVTs) purified from first- trimester placentas. Two-dimension (2D)-differential in-gel electrophoresis analyses revealed that 731 spots were significantly differentially regulated by IL11 in HTR8/SVneo cells: seven spots were analyzed by liquid chromatography-tandem mass spectrometry and 14 unique proteins identified. Protein disulfide isomerase family A, member 3 (PDIA3; endoplasmic reticulum p57) and glucose-regulated protein 78 (GRP78) were further validated to be regulated by IL11 in HTR8/SVneo and primary EVT. One dimension western blot analysis confirmed that PDIA3 was down-regulated in EVT. 2D western blot analysis revealed that GRP78 was post-translationally modified following IL11 treatment. Moreover, IL11 stimulated the secretion of GRP78 in EVT. CONCLUSIONS Data suggest that IL11, possibly via signal transducers and activators of transcription 3 signaling pathway, regulates PDIA3 protein expression and modification/secretion of GRP78. This is the first study to identify PDIA3 and GRP78 as IL11 targets in invasive trophoblasts and identifies a possible mechanism by which IL11 regulates trophoblast function.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali