Quantitation of [5-CH3]-(2R, 4'R, 8'R)-a-Tocopherol in Humans  

Oleh:

Chuang, Jennifer C. ; Matel, Hosea D. ; Nambiar, Krishnan P.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: JN: The Journal of Nutrition vol. 141 no. 08 (Aug. 2011), page 1482-1488 .
Topik: Nutrient Requirements; Optimal Nutrition

Ketersediaan

Perpustakaan FK Nomor Panggil: J42.K.2011.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Half-lives of a-tocopherol in plasma have been reported as 2–3 d, whereas the Elgin Study required >2 y to deplete a-tocopherol, so gaps exist in our quantitative understanding of human a-tocopherol metabolism. Therefore, 6 men and 6 women aged 27 ± 6 y (mean ± SD) ingested 1.81 nmol, 3.70 kBq of [5-14CH3]-(2R, 4'R, 8'R)-a-tocopherol. The levels of 14C in blood plasma and washed RBC were monitored frequently from 0 to 460 d while the levels of 14C in urine and feces were monitored from 0 to 21 d. Total fecal elimination (fecal + metabolic fecal) was 23.24 ± 5.81% of the 14C dose, so feces over urine was the major route of elimination of the ingested [5-14CH3]-(2R, 4'R, 8'R)-a-tocopherol, consistent with prior estimates. The half-life of a-tocopherol varied in plasma and RBC according to the duration of study. The minute dose coupled with frequent monitoring over 460 d and 21 d for blood, urine, and feces ensured the [5-14CH3]-(2R, 4'R, 8'R)-a-tocopherol (the tracer) had the chance to fully mix with the endogenous [5-14CH3]-(2R, 4'R, 8'R)-a-tocopherol (the tracee). The 14C levels in neither plasma nor RBC had returned to baseline by d 460, indicating that the t1/2 of [5-CH3]-(2R, 4'R, 8'R)-a-tocopherol in human blood was longer than prior estimates.

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