Immobilization of Saccharomyces Cerevisiae in Rice Hulls for Ethanol Production  

Oleh:

Martini, Edita

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Makara Journal of Technology vol. 14 no. 2 (Nov. 2010), page 61-64.
Topik: Ethanol; Fermentation; Immobilization; rice Hulls; Yeast Cells

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM64.4 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

The whole cell immobilization in ethanol fermentation can be done by using natural carriers or through synthetic carries. All of these methods have the same purpose of retaining high cell concentrations within a certain defined region of space which leads to higher ethanol productivity. Lignocellulosic plant substance represents one og highly potential sources in ethanol production. Some studies have found that cellulosic substances can also be used as a natural carrier in cell immobilization by re-circulating pre-culture medium into a reactor. In this experiment, rice huls without any treatment were used to immobilize Saccharomyces cerevisiae through semi solid state incubation combined with re-circulating pre-culture medium. The scanning electron mocroscopy (SEM) pictures to the carrier show that the yeast cells are absobed and embedded to the rice hull pore. In liquid batch fermentation system with an initial sugar concentration o 50 g/L, nearly 100% total sugar was consumed after 48 hour. Tis resulted in an ethanol yield of 0.32 g ethanol/g glucose, which is 62.7% of the theoretical value. Ethanol productivity of 0.59 g/(L.h) is 2.3 fold higher than that of free cells which is 0.26 g/(L.h). An effort to reuse the immobilized cells in liquid fermentation system showed poor results due to cell desorption in the firs batch which led to high sugar concentration inhibitory effect in the second batch fermentation. This might be solved by using semi solid fermentation process in the future work.

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